Johns Hopkins Mouse Pathobiology and Phenotyping Short Course
♦ Rigor and Reproducibility in Translational Research 


July 13-17, 2020 – ONLINE !!

REGISTRATION REQUIRED (Click ⇒ Links for registration forms; email completed forms to


⇒ Registration Form for ALL JHU registrants (JHU GRAD STUDENTS, POSTDOCS, FACULTY, STAFF, CORES)

⇒ GRAD students for full credit would schedule in-lab training at a later date.

⇒ Tuition Remission Form (for JHU Faculty & Staff :–$600  $100 for any/all Lectures + Lab Demo Sessions,
      labs, tours, slide conference, Monday reception
⇒ NON JHU Registration Form: $100 for Lectures + Lab Demo Sessions

      Until June 15, 2020: $600. for lectures only; $800. For Lectures + 4 hands on laboratory sessions.
      After June 15 CALL for AVAILABILITY: $800. for lectures only; $1000. For lectures + labs (space permitting).

⇒  EXHIBIT Registration Form  July 13-14 Turner Concourse (includes access to course lectures activities throughout the week)


Target Audience: Graduate students and postdocs, as well as research faculty and staff, who currently work with, or expect to work with mouse models and genetically engineered mice.

Since 2010 this has been offered in a short course format, largely for continuity of the practical laboratory training experience.
In 2020 we are offering lectures and lab demo sessions online, and will be trying some online interactive sessions as well.

Faculty from JHU School of Medicine and Bloomberg School of Public Health introduce concepts in mouse biology, pathology, genetics, normal ‘phenotypes’, phenotyping concepts, new and established phenotyping capabilities in multiple disciplines.
JHU cores and resources are highlighted. See previous course programs/schedules below.

>35 contact hours: ~11hr Laboratory sessions; ~26 hr Lectures; demos, interactive sessions.
33hr Approved RACE CE credit. [Only sessions attended can be claimed for credit]
Four (4) hands-on laboratory sessions (~11 hours total)  include clinical and physical examination of mice, specimen collection, clinical pathology and anatomic pathology
(Familiarity with basic anatomy is EXPECTED for participation in laboratory sessions).

Syllabus material is provided:   Hard copy laboratory manual is provided to all enrolled in laboratory sessions.  Lecture and laboratory syllabus material is provided electronically as pdf’s linked to the program.   It is NOT necessary to purchase a text book.

(JHUSOM graduate school ME 680.712) Course credit and grades are based on:

  1. Attendance and participation in online sessions;
  2. Exam (‘take home’, Open book, JHU honor code applies), distributed Monday at the beginning of the course, and Due Friday, 5pm.The exam is intended to be practical and relevant to diverse research.
  3.  IN LAB training to be scheduled at a later date, after passing the exam.

Course Co-Directors: Cory Brayton, DVM, Dip. ACLAM, ACVP, Julie Watson, MA, VetMB, Dip ACLAM, Kinta Diven, MS.

Laboratory Coordinator: Nadine Forbes McBean, MS –

Course Administrator: Wendy Elza – – 410 502 3050
Graduate students and postdocs in JHU institutions OTHER THAN SOM should contact Wendy for assistance.

PRELIMINARY PROGRAM [confirmed speakers/times are BOLDED]
UPDATES will be posted
Expect 5-10 min breaks between sessions to sort technology issues…

MONDAY            July 13, 2020           Welcome, Intro, Posters, Exhibits

8am-5pm             Registration – Turner Concourse

8am    password protected login will begin

845-10am            Brayton  – Introduction: Phenotyping & Famous Mice

10-11    Brayton  –  Common Phenotypes & Disease (Spontaneous /NON infectious)

11-12    Brayton –  External Environmental (X) Factors and Reproducibility


1-2        C Zeiss – COVID in MICE: options and opportunities

2-3        Bayton–  Microbial & infectious Research Challenges

3-4        Brayton  –  Immunovariability in (immune sufficient and immune deficient) mice

4-5        Hutchinson – Research relevant social life and behaviors of mice



TUESDAY           July 14, 2020          GEM & Multidisciplinary Phenotyping

9-10        Hawkins/Reeves – Making Mice – traditional and new transgenic technologies

10-11      Aja – Metabolic Phenotypes and Phenotyping

11-12      Mitzner – Pulmonary/Respiratory Phenotypes & Phenotyping

CSMR (metabolic), MRB Behavioral, ESC, OTS

130-230   Doyle – Ophthalmic Phenotypes and Phenotyping

230-330   Screven/Lauer – Auditory Phenotypes and Phenotyping

330-430   Foss/Gabrielson  – IMAGING for Phenotyping


WEDNESDAY    July 15, 2020          Practical Phenotyping 1

JH Phenotyping Laboratory Manual 5th ed 2019  (PDF 58p  ~6MB) free to download
REVIEW by Wednesday AM

9-1030    LAB 1: Brayton Villano Forbes et al – Clinical / Physical Exam + DEMO

1030-12  Brayton – Clinical Pathology (Hematology Chemistry, etc for research)

CSMR (metabolic), AIMS Core, MRB Imaging 1230-50, ESC, OTS

130-3    LAB 2: Villano Stover Castell et al Specimen collections and common procedures 

3-4        VIRTUAL Faculty/Core breakout Q&A sessions


THURSDAY       July 16, 2020           Practical Phenotyping 2

9-1030       Brayton/Forbes- Anatomic Pathology Phenotyping  – Necropsy, examination, tissue collection trimming Lecture

1030-1130  LAB 3: Forbes / Brayton/ Myers /Powers Anatomic Pathology – Necropsy, examinaton, tissue collection DEMO/video Q&A

1130-12      OTS CORE Histology Lab

12-1           LUNCH BREAK (on your own)

1-215         Brayton – Comparative Anatomy/Pathology (human/mouse)

230-330     Brayton – Research Relevant Mouse Phenotypes & Pathology – interactive REVIEW (Quiz format)

330-4         Faculty Discussion of participants research questions


FRIDAY             July 17, 2020           Practical Phenotyping 3 Advanced Pathology

9-10       Meeker  –  Immunohistochemistry – principles and protocol development

10-11     A Michel – Digital Pathology: options for image analysis and quantitative pathology   

11-12     E Edmondson – NCI Pathology in Preclinical Cancer Research

12-1      J Ward – Embryo and Developmental Phenotyping

5pm        Quizzes Due ! for course ME680.712

Phenotyping Core
Johns Hopkins Phenotyping and Comparative Pathology Core (MRB)
iLAB information and submission

Animal Care and Use Committee (ACUC)

Applied Imaging Mass Spectrometry (AIMS) Core


Behavioral Core School of Medicine
Rodent Behavioral Phenotyping (MRB)


CSMR Centralized Services for Metabolism Research
Indirect calorimetry, food intake (Rangos)
ES Cell and Transgenic Core
Johns Hopkins Transgenic and ES Cell core


MRB Molecular Imaging Service Center and Cancer Functional Imaging Core
MRI, PET, CT, SPECT, IVIS, Optical Imaging, Ultrasound (MRB)

OTS Oncology Tissue Services Core
TMA, IHC, Slide Scanning, Histology core labs at SKCCC
iLAB information and submission

Laboratory Manual and Previous Programs



Radaelli, Santagostino, Sellers, Brayton. 2019. Immune Relevant and Immune Deficient Mice: Options and Opportunities in Translational Research. IlarJ.