ME:680.712 Phenotyping for Functional Genetics

Johns Hopkins Mouse Pathobiology and Phenotyping Short Course
♦ Rigor and Reproducibility in Translational Research

ME680.712

July 11 -15, 2022- ONLINE !!

REGISTRATION REQUIRED (Click ⇒ Links for registration forms; email completed forms to Welza1@jhmi.edu)

REGISTRATION DEADLINE JULY 6, 2022

⇒ Registration Form for ALL JHU registrants (JHU GRAD STUDENTS, POSTDOCS, FACULTY, STAFF, CORES)

⇒ GRAD students for full credit would schedule in-lab training at a later date.

⇒ Tuition Remission Form (for JHU Faculty & Staff) –$50 for any/all Lectures + Lab Demo Sessions,
labs, tours, slide conference, Monday reception

⇒ NON JHU Registration Form: $50 for Lectures + Lab Demo Sessions

⇒ EXHIBIT Registration (includes access to course lectures activities throughout the week) email welza1@jhmi.edu to get more information

⇒ CONTACT cbrayton@jhmi.edu FOR ONLINE OPTIONS

Target Audience: Graduate students and postdocs, as well as research faculty and staff, who currently work with, or expect to work with mouse models and genetically engineered mice.

Since 2010 this has been offered in a short course format, largely for continuity of the practical laboratory training experience.
In 2021 we are offering lectures and lab demo sessions online, and will be trying some online interactive sessions as well.

Faculty from JHU School of Medicine and Bloomberg School of Public Health introduce concepts in mouse biology, pathology, genetics, normal ‘phenotypes’, phenotyping concepts, new and established phenotyping capabilities in multiple disciplines.
JHU cores and resources are highlighted. See previous course programs/schedules below.

Up to 29 hr Approved RACE CE credit. [Only sessions attended can be claimed for credit]
In lab training to be scheduled at a later date, after passing exam to include clinical and physical examination of mice, specimen collection, clinical pathology and anatomic pathology
(Familiarity with basic anatomy is EXPECTED for participation in laboratory sessions).

Syllabus material is provided: Hard copy laboratory manual is provided to all enrolled in laboratory sessions. Lecture and laboratory syllabus material is provided electronically as pdf’s linked to the program. It is NOT necessary to purchase a text book.

(JHUSOM graduate school ME 680.712) Course credit and grades are based on:

Attendance and participation in online sessions;
Exam (‘take home’, Open book, JHU honor code applies), distributed Monday at the beginning of the course, and Due Friday, 5pm.The exam is intended to be practical and relevant to diverse research.
IN LAB training to be scheduled at a later date, after passing the exam.

Course Co-Directors: Cory Brayton, DVM, Dip. ACLAM, ACVP, Jason Villano, DVM, MS, DACLAM, Kinta Diven, MS.

Course Administrator: Wendy Elza – Welza1@jhmi.edu – 410 502 3050
Graduate students and postdocs in JHU institutions OTHER THAN SOM should contact Wendy for assistance.

REGISTRATION & INFO: https://mcp.bs.jhmi.edu/me680-712-phenotyping-for-functional-genetics/

PRELIMINARY PROGRAM [confirmed speakers/times are BOLDED]
UPDATES will be posted
Expect 5-10 min breaks between sessions to sort technology issues…

MONDAY July 11, 2022 Welcome, Introductions

8am password protected login will begin

845-10am Brayton – Introduction: Phenotyping & Famous Mice

10-11 Brayton – Common Phenotypes & Disease (Spontaneous /NON infectious)

11-12 Brayton – External Environmental (X) Factors and Reproducibility

12-1 LUNCH – BREAKOUT SESSIONS: MEET THE PARTICIPANTS

1-2 C. Zeiss- COVID in MICE: options and opportunities

2-3 Bayton– Microbial & infectious Research Challenges

3-4 Brayton – Immunovariability in (immune sufficient and immune deficient) mice

4-5 Hutchinson – Research relevant social life and behaviors of mice

5-6pm VIRTUAL RECEPTION BYOB: MEET THE PARTICIPANTS

TUESDAY July 12, 2022 GEM & Multidisciplinary Phenotyping

9-10 Hawkins/Reeves – Making Mice – traditional and new transgenic technologies

10-11 Aja – Metabolic Phenotypes and Phenotyping

11-12 Mitzner – Pulmonary/Respiratory Phenotypes & Phenotyping

12-130 LUNCH & VIRTUAL Meet the Cores BREAKOUT SESSIONS
CSMR (metabolic), MRB Behavioral, ESC, OTS

130-230 Doyle – Ophthalmic Phenotypes and Phenotyping

230-330 Vicencio/Lauer – Auditory Phenotypes and Phenotyping

330-430 Foss/Gabrielson – IMAGING for Phenotyping

WEDNESDAY July 13, 2022 Practical Phenotyping 1

JH Phenotyping Laboratory Manual 5th ed 2019 (PDF 58p ~6MB) free to download
REVIEW by Wednesday AM

9-1030 LAB 1: Brayton Villano Forbes et al – Clinical / Physical Exam + DEMO

1030-12 Brayton – Clinical Pathology (Hematology Chemistry, etc for research)

12-130 LUNCH & VIRTUAL Meet the Cores BREAKOUT SESSIONS
CSMR (metabolic), AIMS Core, MRB Imaging 1230-50, ESC, OTS

130-3 LAB 2: Villano Stover Castell et al Specimen collections and common procedures

3-4 VIRTUAL Faculty/Core breakout Q&A sessions AND/OR interactive REVIEW (Quiz format)

THURSDAY July 14, 2022 Practical Phenotyping 2

9-1030 Brayton/Forbes- Anatomic Pathology Phenotyping – Necropsy, examination, tissue collection trimming Lecture

1030-1130 LAB 3: Forbes / Brayton/ Myers /Powers Anatomic Pathology – Necropsy, examinaton, tissue collection DEMO/video Q&A

1130-12 OTS CORE Histology Lab

12-1 LUNCH BREAK (on your own)

1-215 Brayton – Comparative Anatomy/Pathology (human/mouse)

230-330 Meeker – Immunohistochemistry – principles and protocol development

330-430 Faculty Discussion of participants research questions AND/OR Brayton – Research Relevant Mouse Phenotypes & Pathology – interactive REVIEW (Quiz format)

FRIDAY July 15, 2022 Practical Phenotyping 3 Advanced Pathology

9-10 TBD – Brayton – Research Relevant Mouse Phenotypes & Pathology – interactive REVIEW (Quiz format)

10-11 D. Rao – Practical Pre GLP Pathology (efficacy/safety assessments in academic settings)

11-12 L Bassel NCI – Digital Pathology: options for image analysis and quantitative pathology

12-1 J Ward – Embryo and Developmental Phenotyping

1-130 pathology Faculty Discussion of participants research questions

5pm Quizzes Due ! for course ME680.712

PARTICIPATING CORES

Phenotyping Core
Johns Hopkins Phenotyping and Comparative Pathology Core (MRB)
iLAB information and submission https://johnshopkins.corefacilities.org/

Animal Care and Use Committee (ACUC)

Applied Imaging Mass Spectrometry (AIMS) Core

Behavioral Core School of Medicine
Rodent Behavioral Phenotyping (MRB)

CSMR Centralized Services for Metabolism Research
Indirect calorimetry, food intake (Rangos) https://www.hopkinsmedicine.org/institute_basic_biomedical_sciences/research_centers/metabolism_obesity_research/coordinated-services-for-metabolism-research

ES Cell and Transgenic Core
Johns Hopkins Transgenic and ES Cell core https://www.hopkinsmedicine.org/core/Home.htm

MRB Molecular Imaging Core
MRI, PET, CT, SPECT, IVIS, Optical Imaging, Ultrasound (MRB) https://www.hopkinsmedicine.org/radiology/research/service-cores/mrb-service-center-lab/index.html

OTS Oncology Tissue Services Core
TMA, IHC, Slide Scanning, Histology core labs at SKCCC
iLAB information and submission https://johnshopkins.corefacilities.org/

Laboratory Manual and Previous Programs

ADDITIONAL MATERIALS RESOURCES

Radaelli, Santagostino, Sellers, Brayton. 2019. Immune Relevant and Immune Deficient Mice: Options and Opportunities in Translational Research. IlarJ. https://www.ncbi.nlm.nih.gov/pubmed/31197363

Johns Hopkins Mouse Pathobiology and Phenotyping Short Course ♦ Rigor and Reproducibility in Translational Research