Johns Hopkins Mouse Pathobiology and Phenotyping Short Course
♦ Rigor and Reproducibility in Translational Research
ME680.712
July 13-17, 2020 – ONLINE !!
REGISTRATION REQUIRED (Click ⇒ Links for registration forms; email completed forms to Welza1@jhmi.edu)
REGISTRATION DEADLINE JULY 7, 2020
⇒ Registration Form for ALL JHU registrants (JHU GRAD STUDENTS, POSTDOCS, FACULTY, STAFF, CORES)
⇒ GRAD students for full credit would schedule in-lab training at a later date.
⇒ Tuition Remission Form (for JHU Faculty & Staff :–$600 $100 for any/all Lectures + Lab Demo Sessions,
labs, tours, slide conference, Monday reception
⇒ NON JHU Registration Form: $100 for Lectures + Lab Demo Sessions
Until June 15, 2020: $600. for lectures only; $800. For Lectures + 4 hands on laboratory sessions.
After June 15 CALL for AVAILABILITY: $800. for lectures only; $1000. For lectures + labs (space permitting).
⇒ EXHIBIT Registration Form July 13-14 Turner Concourse (includes access to course lectures activities throughout the week)
⇒ CONTACT cbrayton@jhmi.edu FOR ONLINE OPTIONS
Target Audience: Graduate students and postdocs, as well as research faculty and staff, who currently work with, or expect to work with mouse models and genetically engineered mice.
Since 2010 this has been offered in a short course format, largely for continuity of the practical laboratory training experience.
In 2020 we are offering lectures and lab demo sessions online, and will be trying some online interactive sessions as well.
Faculty from JHU School of Medicine and Bloomberg School of Public Health introduce concepts in mouse biology, pathology, genetics, normal ‘phenotypes’, phenotyping concepts, new and established phenotyping capabilities in multiple disciplines.
JHU cores and resources are highlighted. See previous course programs/schedules below.
>35 contact hours: ~11hr Laboratory sessions; ~26 hr Lectures; demos, interactive sessions.
33hr Approved RACE CE credit. [Only sessions attended can be claimed for credit]
Four (4) hands-on laboratory sessions (~11 hours total) include clinical and physical examination of mice, specimen collection, clinical pathology and anatomic pathology
(Familiarity with basic anatomy is EXPECTED for participation in laboratory sessions).
Syllabus material is provided: Hard copy laboratory manual is provided to all enrolled in laboratory sessions. Lecture and laboratory syllabus material is provided electronically as pdf’s linked to the program. It is NOT necessary to purchase a text book.
(JHUSOM graduate school ME 680.712) Course credit and grades are based on:
- Attendance and participation in online sessions;
- Exam (‘take home’, Open book, JHU honor code applies), distributed Monday at the beginning of the course, and Due Friday, 5pm.The exam is intended to be practical and relevant to diverse research.
- IN LAB training to be scheduled at a later date, after passing the exam.
Course Co-Directors: Cory Brayton, DVM, Dip. ACLAM, ACVP, Julie Watson, MA, VetMB, Dip ACLAM, Kinta Diven, MS.
Laboratory Coordinator: Nadine Forbes McBean, MS – nforbes1@jhmi.edu
Course Administrator: Wendy Elza – Welza1@jhmi.edu – 410 502 3050
Graduate students and postdocs in JHU institutions OTHER THAN SOM should contact Wendy for assistance.
PRELIMINARY PROGRAM [confirmed speakers/times are BOLDED]
UPDATES will be posted
Expect 5-10 min breaks between sessions to sort technology issues…
MONDAY July 13, 2020 Welcome, Intro, Posters, Exhibits
8am-5pm Registration – Turner Concourse
8am password protected login will begin
845-10am Brayton – Introduction: Phenotyping & Famous Mice
10-11 Brayton – Common Phenotypes & Disease (Spontaneous /NON infectious)
11-12 Brayton – External Environmental (X) Factors and Reproducibility
12-1 LUNCH – POSTERS, BREAKOUT SESSIONS: MEET THE PARTICIPANTS
1-2 C Zeiss – COVID in MICE: options and opportunities
2-3 Bayton– Microbial & infectious Research Challenges
3-4 Brayton – Immunovariability in (immune sufficient and immune deficient) mice
4-5 Hutchinson – Research relevant social life and behaviors of mice
5-6pm VIRTUAL RECEPTION BYOB: MEET THE PARTICIPANTS
TUESDAY July 14, 2020 GEM & Multidisciplinary Phenotyping
9-10 Hawkins/Reeves – Making Mice – traditional and new transgenic technologies
10-11 Aja – Metabolic Phenotypes and Phenotyping
11-12 Mitzner – Pulmonary/Respiratory Phenotypes & Phenotyping
12-130 LUNCH & VIRTUAL Meet the Cores BREAKOUT SESSIONS
CSMR (metabolic), MRB Behavioral, ESC, OTS
130-230 Doyle – Ophthalmic Phenotypes and Phenotyping
230-330 Screven/Lauer – Auditory Phenotypes and Phenotyping
330-430 Foss/Gabrielson – IMAGING for Phenotyping
WEDNESDAY July 15, 2020 Practical Phenotyping 1
JH Phenotyping Laboratory Manual 5th ed 2019 (PDF 58p ~6MB) free to download
REVIEW by Wednesday AM
9-1030 LAB 1: Brayton Villano Forbes et al – Clinical / Physical Exam + DEMO
1030-12 Brayton – Clinical Pathology (Hematology Chemistry, etc for research)
12-130 LUNCH & VIRTUAL Meet the Cores BREAKOUT SESSIONS
CSMR (metabolic), AIMS Core, MRB Imaging 1230-50, ESC, OTS
130-3 LAB 2: Villano Stover Castell et al Specimen collections and common procedures
3-4 VIRTUAL Faculty/Core breakout Q&A sessions
THURSDAY July 16, 2020 Practical Phenotyping 2
9-1030 Brayton/Forbes- Anatomic Pathology Phenotyping – Necropsy, examination, tissue collection trimming Lecture
1030-1130 LAB 3: Forbes / Brayton/ Myers /Powers Anatomic Pathology – Necropsy, examinaton, tissue collection DEMO/video Q&A
1130-12 OTS CORE Histology Lab
12-1 LUNCH BREAK (on your own)
1-215 Brayton – Comparative Anatomy/Pathology (human/mouse)
230-330 Brayton – Research Relevant Mouse Phenotypes & Pathology – interactive REVIEW (Quiz format)
330-4 Faculty Discussion of participants research questions
FRIDAY July 17, 2020 Practical Phenotyping 3 Advanced Pathology
9-10 Meeker – Immunohistochemistry – principles and protocol development
10-11 A Michel – Digital Pathology: options for image analysis and quantitative pathology
11-12 E Edmondson – NCI Pathology in Preclinical Cancer Research
12-1 J Ward – Embryo and Developmental Phenotyping
5pm Quizzes Due ! for course ME680.712
PARTICIPATING CORES
Phenotyping Core
Johns Hopkins Phenotyping and Comparative Pathology Core (MRB)
iLAB information and submission https://johnshopkins.corefacilities.org/
Animal Care and Use Committee (ACUC)
Applied Imaging Mass Spectrometry (AIMS) Core
Behavioral Core School of Medicine
Rodent Behavioral Phenotyping (MRB)
CSMR Centralized Services for Metabolism Research
Indirect calorimetry, food intake (Rangos)
ES Cell and Transgenic Core
Johns Hopkins Transgenic and ES Cell core https://www.hopkinsmedicine.org/core/Home.htm
MRB Molecular Imaging Service Center and Cancer Functional Imaging Core
MRI, PET, CT, SPECT, IVIS, Optical Imaging, Ultrasound (MRB)
OTS Oncology Tissue Services Core
TMA, IHC, Slide Scanning, Histology core labs at SKCCC
iLAB information and submission https://johnshopkins.corefacilities.org/
Laboratory Manual and Previous Programs
-
JH Phenotyping Laboratory Manual 5th ed 2019 (PDF 58p ~6MB)
- 2019 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2016 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2015 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2013 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2012 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2011 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2010 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2008 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2007 Schedule of Lecture and Laboratory Topics and Faculty (PDF)
- 2006 Phenotyping Symposium (PDF)
- 2007 Phenotyping Symposium (PDF)
- 2008 Phenotyping Symposium (PDF)
- 2009 Phenotyping Symposium (PDF)
ADDITIONAL MATERIALS RESOURCES
Radaelli, Santagostino, Sellers, Brayton. 2019. Immune Relevant and Immune Deficient Mice: Options and Opportunities in Translational Research. IlarJ. https://www.ncbi.nlm.nih.gov/pubmed/31197363