Pathology Services Available From The Phenotyping Core
The Phenotyping Core provides many services to support translational animal research at the Johns Hopkins University. Below is a list of those available and the relevant personnel to contact for further information. Please note that some large scale or intellectually-involved projects may merit grant support or collaboration of the participating faculty member. To officially schedule a service request, please use the iLAB System at JHU (note: JHED login is required). For behavioral, matabolic, and in vivo imaging services, please refer to our list of In Vivo Tests.
Necropsy and Histopathology
Service
Contact
Fee
Notes
Pathology Consulting: Includes diagnosis, analysis, scoring, and photomicroscopy, summary evaluations as well as assistance with publication, presentation, or grant preparation.
Cory Brayton
Free inital consult (up to 1 hour), then $20 per slide or $200 per hour thereafter.
Slides or specimens will be evaluated on a limited basis until a contract or collaborative grant can be established.
Pathology Project: Includes pre-clinical research, phenotyping, or GLP testing.
Cory Brayton
Free inital consult (up to 1 hour), then $20 per slide or $50 for partial necropsy.
Service available on a limited basis until a contract or collaborative grant can be established.
Basic Necropsy (Mouse): Includes gross and histopathology report for <10 slides.
Cory Brayton
$100 per mouse, in addition to histology charges.
Histology processing charged separately (see below).
Comprehensize Baseline Necropsy (Mouse): Includes necropsy, CBC, and serum banking (if available). Histopathology includes examination of 10 slides containing over 40 different tissues.
Cory Brayton
$200 per mouse, in addition to histology charges.
Histology processing charged separately (see below).
Perfusion Fixation: Mice are perfused with neutral-buffered formalin or Bouins Solution prior to necropsy (improves quality of histology).
Nadine Forbes McBean, Cory Brayton
$50 per hour or $10 surcharge to cost of Necropsy (see above).
Must be performed in the Phenotyping Core in MRB. Training is available.
Special Pathology Techniques: Includes difficult dissections, perfusion, and training in the above.
Nadine Forbes McBean, Cory Brayton
$50 per hour (minimum $50).
Training available.
Photography: Publication-quality digital photography of whole animals or organs.
Nadine Forbes McBean, Cory Brayton
$50 per hour (minimum $50).
Stereomicropscopy (up to 10× magnification) available. Please specify digital format and desired resolution.
Histologic Sectioning: Includes paraffin embedding of tissues, sectioning, and routine staining of slides.
Pat Wilcox
Variable, see price list.
Digital Slide Scanning: Digitization, archival and image analysis of whole slides.
JHU Oncology Tissue Services
Variable, see price list.
Tissue Microarrays: Choose from pre-built arrays or design a custom tissue microarray for medium-throughout analysis.
JHU Oncology Tissue Services
Variable, see price list.
Immunohistochemistry: Choose from pre-built arrays or design a custom tissue microarray for medium-throughout analysis.
JHU Oncology Tissue Services
Variable, see price list.
Request a service via the JHU iLAB System
Clinical Pathology
Service
Contact
Fee
Notes
Phlebotomy: Blood and serum collection from mice.
Nadine Forbes McBean
$15 per mouse or $50 per hour.
Cost includes sample tubes and other disposables.
Hematology or Cytology Specimen Staining: Includes the DifQuik Modified Wright’s stain by default. Other methods available upon request.
Nadine Forbes McBean
$15 per mouse and $50 per hour.
Please contact us BEFORE submitting samples.
Hematology or Cytology Interpretation: Evaluation of blood or impression smear, bone marrow specimen, or fluid specimen (effusion, urine, etc.).
Cory Brayton
$20 per stained slide.
Service available on a limited basis until a contract or collaborative grant can be established.
Cytospin: Concentration of cells from dilute specimens onto glass slides via centifugation.
Nadine Forbes McBean
$10 per slide and $50 per hour plus cost of disposables.
Please contact us BEFORE submitting samples. We cannot accept biohazardous specimens! Cost does not include staining or interpretation.
Hematology with CBC: Includes analysis with Hemavet 950 analyzer and creation of an unstained blood smear.
Nadine Forbes McBean
$12 per sample.
50 μL whole blood with concurrent control samples recommended.
Chemistry Panel: Serum chemistry panel (protein, enzyme, and electrolyte levels) performed on an Alfa Wasserman VetACE chemistry analyzer.
Nadine Forbes McBean
$6 for serum samples or $12 for whole blood specimens.
120 μL whole blood or 60 μL serum or plasma.
Glucometry: Blood glucose measurement with Accucheck or OneTouch glucometer.
Nadine Forbes McBean
$20 per mouse or $50 per hour + $3 per strip.
Requires ~10 μL whole blood. Cage-side testing with concurrent controls is recommended. Training in this procedure is available.
Urinalysis: Includes specific gravity measurement with refractometer; glucose, protein, and blood by dipstick, and microscopic sediment analysis.
Nadine Forbes McBean
$5–$25.
Requires 50–100 μL for most tests.
Pathogen Screening: Includes diagnostic serology, parasitology, microbiology, or PCR for animal pathogens.
Julie Watson
Varies, please enquire.
Please contact Research Animal Resources to report ill mice or other clinical problems.
Additonal Rodent Services: Includes breeding, genotyping, fostering, or special procedures.
Julie Watson
Varies, please enquire.
Please contact Research Animal Resources to report ill mice or other clinical problems.