Phenotyping Core Testing

Pathology and Testing through the Phenotyping Core

Please contact relevant faculty or personnel BEFORE beginning a study or submitting specimens, in order to optimize testing and results.
Formal %FTE salary support is expected for large or long term projects. It is expected that JHU policies for authorship and acknowledgment will be followed for 'fee for service' projects as well as for grant supported projects.
Note that all procedures involving animals should be ACUC approved prior to the procedures.
To schedule testing please use the iLAB system at JHU (note: JHED login is required).

NOTE: NO hazardous, and NO human specimens are tested.

Anatomic Pathology: Necropsy and Histopathology

TESTS /options  
(PDF summary FY 2019-20)
Contact
Costs
Notes
Pathology Consulting: Slide analysis, can include scoring, photomicroscopy, summary evaluations as well as assistance with publication, presentation, or grant preparation. Cory Brayton Free inital consult (up to 1 hour), then $20 per slide or $200 per hour. Available on a limited basis until contract or grant collaboration is established. See also JHU authorship policies.

Pathology Projects: for pre-clinical research, phenotyping. FYI JH Phenotyping Lab Manual 5th ed 2019 (with protocols for mice) 

Cory Brayton Free inital consult (up to 1 hour), then charged by test or by hour. Available on a limited basis until contract or grant collaboration is established. See also JHU authorship policies.
Custom Necropsy (Mouse): gross and histopathology report for <5 slides. Case Info Form  Cory Brayton From $100 per mouse, in addition to histology charges. Available on a limited basis until contract or grant collaboration is established.
Custom Necropsy (non mice): gross and histopathology report, project specific collections, assessments, reporting Cory Brayton Free inital consult (up to 1 hour), then $200 per hour plus charges for additional personnel, tests, histology processing. Available on a limited basis until contract or grant collaboration is established.
Comprehensive Baseline Necropsy (Mouse): Includes necropsy, CBC. Histopathology includes examination of 10 slides (>40 tissues).  Case Info Form  Cory Brayton $200 per mouse, in addition to histology; addl charges may apply for images, summary reports of multiple cases /projects, etc special requests. Available on a limited basis until  contract or grant collaboration is established
Perfusion Fixation (mice): Perfusion fixation (usually 10%NBF) improves quality of histology, and specimens can be retained and evaluated at a later date (potentially years later). Nadine Forbes McBean $75 per hour or $10 surcharge to Necropsy cost (above). Must be performed in the Phenotyping Core in MRB. Training is available.
Special Pathology Techniques: Includes special dissections, perfusion, and training in the above. Cory Brayton $75 per hour (minimum $75). Training available.
Photography: Publication-quality digital photography of whole animals or organs. Nadine Forbes McBean $75 per hour (minimum $75). Stereomicropscopy (up to 10× magnification) available. Please specify digital format and desired resolution.
Photomicroscopy: Publication-quality digital photography of histology slides. Cory Brayton $200 per hour (minimum $200). Relevant controls are recommended. Please specify magnifications, digital format and resolution.
Histology processing: Includes paraffin processing of fixed tissues, sectioning, H&E, and special stains. Pat Wilcox Variable, see price list. Submission of trimmed tissues in cassettes is recommended.
Histology processing; Digital Slide Scanning; Tissue Microarrays; Immunohistochemistry: JHU Oncology Tissue Services Variable, see price list.  

CLICK HERE for Sheduling and Submissions via JHU iLAB System                                   NOTE: NO hazardous, and NO human specimens are tested.

Clinical Pathology

TESTS /options  
(PDF summary FY 2019-20)
Contact
Costs
Notes
Blood collection from mice. Plasma/serum separation is an additional charge, or included in per hour charge. Nadine Forbes McBean $15 per mouse or $75 per hour. As approved in ACUC protocol. Cost includes sample tubes and standard disposables. 
Hematology or Cytology Specimen Staining: DifQuik Modified Wright's stain. Other methods available upon request. Nadine Forbes McBean $15 per mouse and $75 per hour. Please contact us BEFORE submitting samples.
Hematology or Cytology Interpretation: Evaluation of blood or impression smear, bone marrow specimen, or fluid specimen (effusion, urine, etc.). Cory Brayton $20 per stained slide. Service available on a limited basis until a contract or collaborative grant can be established.
Cytospin: Concentration of cells from dilute specimens onto glass slides via centifugation. Nadine Forbes McBean $10 per slide and $75 per hour plus cost of disposables. Please contact us BEFORE submitting samples. We cannot accept hazardous or human specimens! Cost does not include staining or interpretation.
Hematology: CBC by Procyte automated analyzer; and unstained blood smear. ClinPath Case Submission Info Nadine Forbes McBean $12 per sample. 60 μL whole blood with concurrent control samples recommended. (FYI mouse CBC information)
Chemistry Panel: Serum chemistry panel (protein, enzyme, and electrolyte levels) performed on an Alfa Wasserman VetACE or Diasys Respons 910 chemistry analyzer. ClinPath Case Submission Info Nadine Forbes McBean FROM $6/test for serum samples or $12 for whole blood specimens.

60-500  μL serum or plasma depending on tests/ panels - more info at PDF summary FY 2019-20

Glucometry: Blood glucose measurement with Accucheck or OneTouch glucometer. Nadine Forbes McBean $20 per mouse or $50 per hour + $3 per strip. Requires ~10 μL whole blood. Cage-side testing with concurrent controls is recommended. Training in this procedure is available.
Urinalysis: SG specific gravity measurement by refractometer; SG, glucose, protein, blood by dipstick; microscopic sediment analysis. Nadine Forbes McBean $5–$25. 50–100 μL required for most tests.

 CLICK HERE for Sheduling and Submissions via JHU iLAB System                                   NOTE: NO hazardous, and NO human specimens are tested.

Behavioral Testing

TESTS
Contact
Costs
Notes
Clinical/Physical Exam: Includes SHIRPA-type evaluation of sensory and motor phenotypes. Nadine Forbes McBean $75 per hour. Available cage-side or in lab. Requires appropriate controls. Training available.
Acoustic/Auditory Phenotyping: Assessment of hearing function in mice and rats. Amanda Lauer Varies, please enquire. Collaboration may be preferred or required. Requires appropriate control animals.
Behavioral Assessment: Available for mice and rats. Mikhail Pletnikov Varies, please enquire. Collaboration may be preferred or required. In vivo testing in MRB facilities. Requires appropriate control. SOM MRB Behavior Core.
CLICK HERE for Sheduling and Submissions via JHU iLAB System                                   NOTE: NO hazardous, and NO human specimens are tested.

Imaging, Metabolic, and Pathogen Testing

TESTS /options  
(PDF summary FY 2019-20)
Contact
Fee
Notes
Body Composition: Quantification of body fat, lean mass, and water with EchoMRI 100 QNMR instrument. Nadine Forbes McBean $75 per hour. In vivo etc testing in MRB facilities. Requires appropriate controls.
Faxitron Radiography: High resolution "flat film" radiography for small rodents. Nadine Forbes McBean $75 per hour + $10 per film. In the Core Laboratory. Films can be scanned for archiving and analysis.
Cardiovascular Phenotyping: Imaging, ultrasound, blood pressure, and ECG tests for rodents. Kathleen Gabrielson Varies, please enquire. Collaboration may be preferred or required. In vivo testing in MRB facilities.
Advanced Imaging: MRI, PET, SPEC, CT, and IVIS imaging for rodents. SAIRP Varies, please enquire. Collaboration may be preferred or required. In vivo etc testing in MRB facilities.
Pulmonary Phenotyping: pulmonary function and other testing. Wayne Mitzner Varies, please enquire. Collaboration may be preferred or required. In vivo etc testing.
Mass Spectrometry and Metabolomics: Screening for protein and small metabolite biomarkers. David Graham Varies, please enquire. Collaboration may be preferred or required. On urine, plasma, etc specimens. Center for Resources in Integrative Biology for more details.
Metabolic Phenotyping: Includes calorimetry; O2, food, and water consumption; urinary and/or fecal output measurements. Susan Aja Varies, please enquire.

Centralized Services for Metabolism Research  In vivo etc testing.

Pathogen Screening: Includes diagnostic serology, parasitology, microbiology, or PCR for animal pathogens. Jason Villano Varies, please enquire. Please contact Research Animal Resources to report ill mice or other clinical problems.
Additonal Rodent Services: Includes breeding, genotyping, fostering, or special procedures. Jason Villano Varies, please enquire. Please contact Research Animal Resources to report ill mice or other clinical problems.
CLICK HERE for Sheduling and Submissions via JHU iLAB System                                   NOTE: NO hazardous, and NO human specimens are tested.