Kelly A. Metcalf Pate, DVM, PhD, DACLAM

Kelly A. Metcalf Pate, DVM, PhD, DACLAM

Assistant Professor

410-955-9770

 

Education

BA
DVM
PhD
Boston University, Boston MA
Purdue University, Purdue IN
Johns Hopkins University School of Medicine, Baltimore MD
2003
2007
2013

 

Current Research

Dr. Metcalf Pate’s research focuses on the role of platelets in the innate immune response to viral infection, and how modulating the response of platelets to infection alters the course of disease. Platelets are tiny anuclear cells that outnumber leukocytes in the peripheral blood more than one hundred to one. Platelets are known to participate in innate immunity through cytokine signaling and direct interactions with other cells, and the platelet has the potential to significantly influence disease outcomes. However, platelet immunology is still a relatively new discipline, and the downstream effects of platelet interactions with other immune cells have yet to be determined in the context of viral infection.

The well-characterized and consistent SIV-infected pigtailed macaque model of HIV infection provides an ideal animal model in which to explore the consequences of platelet-leukocyte aggregates during acute infection. Dr. Metcalf Pate has demonstrated that 80% of CD16+ monocytes are bound to platelets during acute SIV infection, while only 6% of this population are bound in mock-inoculated controls. CD16+ monocytes are known to play important roles in the pathogenesis of SIV infection as they are infected more frequently than CD16- monocytes and transmigrate through endothelium early in infection to establish inflammatory foci in organs such as the brain and lungs. Current research aims include further characterization of the platelet-monocyte interaction during acute viral infection with the goal of establishing methods of pharmacologically manipulating this association, and establishing how platelet binding to a monocyte influences the monocyte’s susceptibility to lentiviral infection and the monocyte’s interactions with endothelium.

Dr. Metcalf Pate is additionally interested in the effect of physiologic stress on platelet function, specifically on the platelet’s future immune response to infection, and in the development and optimization of novel in vitro systems that better model in vivo conditions. She welcomes collaborative inquiries from local undergraduates, veterinary students, and researchers with complementary interests.

Current Funded Projects

  • "Comparison of commonly measured immunologic and hemostatic factors in SIV infected macaques following sedated or voluntary awake phlebotomy", ACLAM Foundation Grant
  • “Establishing an EcoHIV mouse model for the study of platelet activation and microbiome towards an HIV cure”, NIH OD R03 OD026691, in collaboration with Dr. Barb Slusher and Dr. Alton Swennes

Current Students

  • Selena Guerrero-Martin, PhD Candidate, Cellular and Molecular Medicine Graduate Program
  • Claire Lyons, DVM, Postdoctoral Fellow
  • Natalie Castell, DVM, Veterinarian Postdoctoral Fellow
  • Shefali Vijay, Undergraduate Researcher, Johns Hopkins University
  • Aileen Feng, Undergraduate Researcher, Johns Hopkins University

 

Current Research Staff

  • Bess Carlson, Research Technologist and Positive Reinforcement Trainer
  • Alisa McNamara, Positive Reinforcement Trainer

 

Former Postdoctoral Students

  • Alicia Braxton, DVM, Veterinarian Postdoctoral Fellow 2016 - 2019, Molecular and Comparative Pathobiology Research Training Program, currently a graduate student in the Cellular and Molecular Medicine program at Johns Hopkins University School of Medicine
  • Casey Kissel, DVM, Veterinarian Postdoctoral Fellow 2016 - 2019,  Molecular and Comparative Pathobiology Laboratory Animal Medicine Training Program, currently a Clinical Research Veterinarian with the Perelman School of Medicine at University of Pennsylvania
  • Meghan Vermillion, DVM, PhD, DACLAM, Veterinarian Postdoctoral Fellow 2013 - 2017, Johns Hopkins University School of Medicine Molecular and Comparative Patholobiology Research Training Program, curently a Veterinary Research Scientist at Lovelace Respiratory Institute
  • Jessica Izzi, DVM, MS, DACLAM, Veterinarian Postdoctoral Fellow 2013 - 2015, Molecular and Comparative Patholobiology Laboratory Animal Medicine Training Program, currently an Assistant Professor at Johns Hopkins University School of Medicine
  • Zachary Freeman, DVM, PhD, DACLAM, Veterinarian Postdoctoral Fellow 2011 - 2015,  Molecular and Comparative Patholobiology Research Training Program, currently an Assistant Professor at University of Michigan School of Medicine
  • Victoria Baxter, DVM, PhD, DACLAM, Veterinarian Postdoctoral Fellow 2010 - 2013,  Molecular and Comparative Patholobiology Research Training Program, currently an Assistant Professor at University of North Carolina Chapel Hill

 

Former Predoctoral Students and Staff

  • Brenna Daly, Summer Boehringer Ingelheim Veterinary Research Scholar 2018 while a veterinary student at Tufts University, currently a veterinary student at Tufts University
  • Kirstin McGee, Undergraduate Researcher 2018-2019, Notre Dame in Maryland, currently a laboratory technologist at Kennedy Krieger Institute
  • Diane Hong, Undergraduate Researcher 2018, Johns Hopkins University
  • Griffin Cyphers, Undergraduate Researcher 2018, Johns Hopkins University
  • Alyssa Chalmin, Undergraduate Researcher 2017-2018, Johns Hopkins University, currently a veterinary student at University of Pennsylvania
  • Wilfred Ikejiofor, Summer Undergraduate Researcher 2017, University of Maryland
  • Paul Moon, Summer High School Researcher 2017, Center for Talented Youth 
  • Karl Johnson, Undergraduate Researcher 2015 - 2017, Johns Hopkins University, currently a PhD student in Public Health at UNC Chapel Hill
  • Liz Engle, Master's Thesis Student 2015 - 2016, Johns Hopkins University Biotechnology Masters Program, currently a Lab Manager at Johns Hopkins School of Medicine Tumor Microenvironment Core
  • Kevin Najarro, Research Technologist 2014 - 2016, currently a Lab Manager at University of Colorado
  • Benjamin Aledejebi, Summer High School Researcher 2015, Center for Talented Youth, currently an undergraduate at Bucknell University
  • Jacqueline Brockhurst, VMD, Summer Merial Veterinary Research Scholar 2015 while a veterinary student at University of Pennysylvania, currently a VMD postdoctoral fellow at Johns Hopkins University School of Medicine
  • Hayley Weidenbenner, Undergraduate Researcher, Johns Hopkins University School of Medicine
  • Claire Lyons, DVM, Undergraduate Researcher 2012 – 2013, Research Technologist 2013 - 2014, currently a DVM postdoctoral fellow at Johns Hopkins University School of Medicine
  • Catherine Cryer, Summer Veterinary Student Researcher 2014 while at University of Pennsylvania School of Veterinary Medicine, currently a veterinarian.
  • Hannah Schneider, Summer Undergraduate Researcher 2013, currently an undergraduate at Colorado State University

 

Want to learn more? Check out

Our platelet-endothelial work on Johns Hopkins University Tomorrow’s Discoveries: https://www.youtube.com/watch?v=ro0pau4qolo&feature=youtu.be

 

Dr. Metcalf Pate’s on being both a veterinarian and a researcher in Johns Hopkins University Fundamentals:

https://www.hopkinsmedicine.org/research/advancements-in-research/fundamentals/profiles/kelly-metcalf-pate-on-being-both-a-vet-and-a-biomedical-researcher

 

Dr. Metcalf Pate on socially housing macaques in infectious disease studies on NPR:

https://www.npr.org/sections/health-shots/2017/10/17/546922313/scientists-push-to-house-more-lab-monkeys-in-pairs

 

The Metcalf Pate Lab Website: https://metcalfpatelab.weebly.com/

 

Selected Publications

  1. Braxton AM, Chalmin AL, Najarro KM, Brockhurst JK, Johnson KT, Lyons CE, Daly B, Cryer CG, Vijay S, Cyphers G, Guerrero-Martin SM, Aston SA, McGee K, Su YP, Arav-Boger R, Metcalf Pate KA. Platelet-endothelial associations may promote cytomegalovirus replication in the salivary gland in mice. Platelets. 2019 Nov 14:1-9. 

  2. Vermillion MS, Lyons CE, Najarro KM, Adams RJ, Metcalf Pate KA. Platelets are immune activated in response to serial phlebotomy in pigtailed macaques (Macaca nemestrina). Comp Med. 2017; 67(4): 360-367

  3. Metcalf Pate KA, Pohlmeyer CW, Walker-Sperling VE, Foote JB, Najarro KM, Cryer CG, Salgado M, Engle EL, Shirk EN, Queen SE, Chioma S, Vermillion ME, Bullock B, Li M, Lyons CE, Adams RJ, Gama L, Zink MC, Clements JE, Mankowski JL, Blankson JN. A murine viral outgrowth assay to detect residual HIV-1 infection. J Infect Dis. 2015; 212(9): 1387-96. PMCID: 25883388

  4. Metcalf Pate KA, Lyons CE, Dorsey JL, Queen SE, Adams RJ, Morrell CN, Mankowski JL. Thrombopoietin downregulation and elevated plasma TGFβ are associated with platelet decline in asymptomatic SIV infection. JAIDS. 2014; 65(5): 510-6. PMCID 24220290

  5. Baxter VK, Shaw GC, Sotuyo NP, Carlson CS, Olson EJ, Zink MC, Mankowski JL, Adams RJ, Hutchinson EK, Metcalf Pate KA. Serum Albumin and Body Weight as Biomarkers for the Antemortem Identification of Bone and Gastrointestinal Disease in the Common Marmoset. PLoS One. 2013; 8(12): e82747. PMCID 24324827

  6. Metcalf Pate KA, Lyons CE, Dorsey JL, Shirk EN, Queen SE, Adams RJ, Gama L, Morrell CN, Mankowski JL. Platelet activation and platelet-monocyte aggregate formation contribute to platelet decline during acute SIV infection in pigtailed macaques. J Infect Dis. 2013; 208(6):874-883. PMCID 23852120


View complete list of publications on PubMed.