ME:680.712 Phenotyping for Functional Genetics

Johns Hopkins Mouse Pathobiology and Phenotyping Short Course
♦ Rigor and Reproducibility in Translational Research 

ME680.712
 

July 13-17, 2020 - ONLINE !!
 

REGISTRATION REQUIRED (Click ⇒ Links for registration forms; email completed forms to Welza1@jhmi.edu
 

REGISTRATION DEADLINE JULY 7, 2020 
 

⇒ Registration Form for ALL JHU registrants (JHU GRAD STUDENTS, POSTDOCS, FACULTY, STAFF, CORES)

⇒ GRAD students for full credit would schedule in-lab training at a later date.

⇒ Tuition Remission Form (for JHU Faculty & Staff :–$600  $100 for any/all Lectures + Lab Demo Sessions,
      labs, tours, slide conference, Monday reception
  
⇒ NON JHU Registration Form: $100 for Lectures + Lab Demo Sessions

      Until June 15, 2020: $600. for lectures only; $800. For Lectures + 4 hands on laboratory sessions.
      After June 15 CALL for AVAILABILITY: $800. for lectures only; $1000. For lectures + labs (space permitting).

⇒  EXHIBIT Registration Form  July 13-14 Turner Concourse (includes access to course lectures activities throughout the week)
⇒  CONTACT cbrayton@jhmi.edu FOR ONLINE OPTIONS

 

Target Audience: Graduate students and postdocs, as well as research faculty and staff, who currently work with, or expect to work with mouse models and genetically engineered mice.

Since 2010 this has been offered in a short course format, largely for continuity of the practical laboratory training experience.
In 2020 we are offering lectures and lab demo sessions online, and will be trying some online interactive sessions as well.

Faculty from JHU School of Medicine and Bloomberg School of Public Health introduce concepts in mouse biology, pathology, genetics, normal 'phenotypes', phenotyping concepts, new and established phenotyping capabilities in multiple disciplines.  
JHU cores and resources are highlighted. See previous course programs/schedules below.

>35 contact hours: ~11hr Laboratory sessions; ~26 hr Lectures; demos, interactive sessions.
33hr Approved RACE CE credit. [Only sessions attended can be claimed for credit]
Four (4) hands-on laboratory sessions (~11 hours total)  include clinical and physical examination of mice, specimen collection, clinical pathology and anatomic pathology
(Familiarity with basic anatomy is EXPECTED for participation in laboratory sessions).

Syllabus material is provided:   Hard copy laboratory manual is provided to all enrolled in laboratory sessions.  Lecture and laboratory syllabus material is provided electronically as pdf's linked to the program.   It is NOT necessary to purchase a text book.

(JHUSOM graduate school ME 680.712) Course credit and grades are based on:

  1. Attendance and participation in online sessions; 
  2. Exam ('take home', Open book, JHU honor code applies), distributed Monday at the beginning of the course, and Due Friday, 5pm.  
    The exam is intended to be practical and relevant to diverse research.      
  3.  IN LAB training to be scheduled at a later date, after passing the exam.

Laboratory Coordinator: Nadine Forbes McBean, MS - nforbes1@jhmi.edu

Course Administrator: Wendy Elza - Welza1@jhmi.edu - 410 502 3050
Graduate students and postdocs in JHU institutions OTHER THAN SOM should contact Wendy for assistance.

PRELIMINARY PROGRAM [confirmed speakers/times are BOLDED] 
UPDATES will be posted 
Expect 5-10 min breaks between sessions to sort technology issues...

MONDAY            July 13, 2020           Welcome, Intro, Posters, Exhibits 
8am-5pm             Registration – Turner Concourse
8am    password protected login will begin 
845-10am            Brayton  – Introduction: Phenotyping & Famous Mice
10-11    Brayton  –  Common Phenotypes & Disease (Spontaneous /NON infectious)
11-12    Brayton –  External Environmental (X) Factors and Reproducibility 
12-1      LUNCH   –  POSTERSBREAKOUT SESSIONS: MEET THE PARTICIPANTS
1-2        C Zeiss - COVID in MICE: options and opportunities
2-3        Bayton–  Microbial & infectious Research Challenges
3-4        Brayton  –  Immunovariability in (immune sufficient and immune deficient) mice
4-5        Hutchinson – Research relevant social life and behaviors of mice
5-6pm   VIRTUAL RECEPTION BYOB: MEET THE PARTICIPANTS 
 
TUESDAY           July 14, 2020          GEM & Multidisciplinary Phenotyping
9-10        Hawkins/Reeves – Making Mice - traditional and new transgenic technologies
10-11      Aja – Metabolic Phenotypes and Phenotyping
11-12      Mitzner – Pulmonary/Respiratory Phenotypes & Phenotyping  
12-130   LUNCH & VIRTUAL Meet the Cores BREAKOUT SESSIONS 
                                 CSMR (metabolic), MRB Behavioral, ESC, OTS
130-230   Doyle – Ophthalmic Phenotypes and Phenotyping
230-330   Screven/Lauer – Auditory Phenotypes and Phenotyping
330-430   Foss/Gabrielson  – IMAGING for Phenotyping
 
WEDNESDAY    July 15, 2020          Practical Phenotyping 1
JH Phenotyping Laboratory Manual 5th ed 2019  (PDF 58p  ~6MB) free to download
REVIEW by Wednesday AM
9-1030    LAB 1: Brayton/Forbes et al - Clinical / Physical Exam + DEMO
1030-12  Brayton - Clinical Pathology (Hematology Chemistry
12-130   LUNCH & VIRTUAL Meet the Cores BREAKOUT SESSIONS 
                                 CSMR (metabolic), AIMS Core, MRB Imaging 1230-50, ESC, OTS
130-3    LAB 2: Villano Stover Castell et al Specimen collections and common procedures 
3-4        VIRTUAL Faculty/Core breakout Q&A sessions
 
THURSDAY       July 16, 2020           Practical Phenotyping 2
9-1030       Brayton/Forbes- Anatomic Pathology Phenotyping  – Necropsy, examination, tissue collection trimming Lecture
1030-1130  LAB 3: Forbes / Brayton/ Myers /Powers Anatomic Pathology – Necropsy, examinaton, tissue collection DEMO/video Q&A 
1130-12      OTS CORE Histology Lab
12-1           LUNCH BREAK (on your own)
1-215         Brayton – Comparative Anatomy/Pathology (human/mouse)
230-330     Brayton – Research Relevant Mouse Phenotypes & Pathology – interactive REVIEW (Quiz format)
330-4         Faculty Discussion of participants research questions
 
FRIDAY             July 17, 2020           Practical Phenotyping 3 Advanced Pathology
9-10       Meeker  –  Immunohistochemistry - principles and protocol development
10-11     A Michel – Digital Pathology: options for image analysis and quantitative pathology   
11-12     E Edmondson – NCI Pathology in Preclinical Cancer Research
12-1      J Ward – Embryo and Developmental Phenotyping 

5pm        Quizzes Due ! for course ME680.712
PARTICIPATING CORES

Phenotyping Core
Johns Hopkins Phenotyping and Comparative Pathology Core (MRB)
iLAB information and submission https://johnshopkins.corefacilities.org/

Animal Care and Use Committee (ACUC)

 
Behavioral Core School of Medicine
Rodent Behavioral Phenotyping (MRB)
  
CSMR Centralized Services for Metabolism Research
Indirect calorimetry, food intake (Rangos)
  
   

MRB Molecular Imaging Service Center and Cancer Functional Imaging Core
MRI, PET, CT, SPECT, IVIS, Optical Imaging, Ultrasound (MRB)

OTS Oncology Tissue Services Core
TMA, IHC, Slide Scanning, Histology core labs at SKCCC
iLAB information and submission https://johnshopkins.corefacilities.org/


Laboratory Manual and Previous Programs

 

ADDITIONAL MATERIALS RESOURCES

Radaelli, Santagostino, Sellers, Brayton. 2019. Immune Relevant and Immune Deficient Mice: Options and Opportunities in Translational Research. IlarJ. https://www.ncbi.nlm.nih.gov/pubmed/31197363